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GENOMIC FEATURES OF COTESIA PLUTELLAE POLYDNAVIRUS
Author(s) -
Cailing LIU,
Xiangxiong ZHU,
Wenjun FU,
Mujun ZHAO
Publication year - 2003
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.2003.tb00371.x
Subject(s) - biology , genome , genomic dna , plasmid , genetics , dna , gene
Polydnavirus was purified from the calyx fluid of Cotesia plutellae ovary. The genomic features of C. plutellae polydnavirus (CpPDV) were investigated. The viral genome consists of at least 12 different segments and the aggregate genome size is a lower estimate of 80kbp. By partial digestion of CpPDV DNA with Bam HI and subsequent ligation with Bam HI‐cut plasmid Bluescript, a representative library of CpPDV genome was obtained.