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STUDY ON THE DIVERSITY OF CYTOCHROME P450 GENES FROM AEDES ALBOPICTUS *
Author(s) -
Guoli ZHOU,
Jionglie HUANG,
Yu WU,
Ling WANG,
Hioian LAO
Publication year - 2001
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.2001.tb00433.x
Subject(s) - biology , gene , aedes albopictus , genetics , complementary dna , homology (biology) , sequence analysis , clone (java method) , rapid amplification of cdna ends , microbiology and biotechnology , aedes aegypti , molecular cloning , botany , larva
Several pairs of specific primers according to the obtained cDNA sequence fragment from deltamethrin‐resistant Aedes albopktus were designed to amplify new CYP6 genes from total RNA of Aedes albopictus by rapid amplification of cDNA ends (RACE) technique. The products of RACE were cloned and selected for sequencing. The deduced amino acid sequences were subjected to homologous analysis. The results indicated that the identities of clone GZS331 sequence from 5′‐RACE products and clone GZG033 sequence from 3′‐RACE products to CYP6N3vl ‐ v3 are 83.9% ‐ 84.3% and 98.2% ‐ 99.1% respectively; while the identities of the others from 3′‐RACE products to CYP6N3v1 ‐ v3 are 84.3% ‐ 85.6%. All of these obtained cDNA sequences have a higher homology to CYP3A1 in mouse and CYP9A1 in moth. The dendrogram constructed by PC/GENE software showed similar results to homologous analysis. These obtained sequences were submitted and named by the P450 Nomenclature Committee. The diversity of cytochrome P450 genes in Culicidae species was discussed.