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Detection of lettuce necrotic yellows virus by an enzyme‐linked immunosorbent assay in plant hosts and the insect vector
Author(s) -
CHU P. W. G.,
FRANCKI R. I. B.
Publication year - 1982
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1982.tb07201.x
Subject(s) - biology , virology , vector (molecular biology) , insect , plant virus , virus , luteovirus , botany , gene , recombinant dna , genetics
SUMMARY An enzyme‐linked immunosorbent assay (ELISA) system for the rapid detection of the plant rhabdovirus, lettuce necrotic yellows virus (LNYV) in plant hosts and individual aphid vector is described. The method has been shown to be reliable and sensitive, and to have a number of advantages over the conventional methods of detecting LNYV in plants and insects by infectivity tests.