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Simple method for determination of oxalic acid in forages using high‐performance liquid chromatography
Author(s) -
Rahman Mohammad Mijanur,
Niimi Mitsuhiro,
Kawamura Osamu
Publication year - 2007
Publication title -
grassland science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.388
H-Index - 19
eISSN - 1744-697X
pISSN - 1744-6961
DOI - 10.1111/j.1744-697x.2007.00093.x
Subject(s) - oxalic acid , oxalate , high performance liquid chromatography , chromatography , chemistry , extraction (chemistry) , column chromatography , sodium carbonate , forage , sodium , agronomy , organic chemistry , biology
We established a simple method for determination of oxalate in forage grasses using high‐performance liquid chromatography (HPLC) with an anion analysis column (Shodex 1C SI‐90 4E) and sodium hydrogen carbonate (NaHCO 3 ) as eluent. Oxalate was determined after extraction with water or 1 mol HCl (soluble form or total, respectively). Insoluble oxalate was estimated as the difference between total oxalates and soluble form. Recovery of oxalate after HPLC was above 97%. Oxalates in forages could be successfully determined by this method. The method was simple, fast and inexpensive.