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IMPAIRED H‐2 EXPRESSION IN B 16 MELANOMA VARIANTS
Author(s) -
Nanni P.,
Colombo M. P.,
Giovanni C. De,
Lollini P.L.,
Nicoletti G.,
Parmiani G.,
Prodi G.
Publication year - 1983
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1983.tb00348.x
Subject(s) - cytotoxic t cell , in vitro , in vivo , effector , cytotoxicity , melanoma , biology , antigen , microbiology and biotechnology , cell culture , b cell , immunology , cancer research , antibody , genetics
SUMMARY We studied the expression of H‐2 b alloantigens in three different B16 melanoma lines cultured in vitro , Cell lines were B16‐F1 and two cell cultures (named B16‐A and B16‐B) newly derived from two different in vivo sublines of B16 melanoma. The assays used were in vivo tumour growth in allogeneic (BALB/c and B10.BR) as compared to syngeneic mice, in vitro cell‐mediated cytotoxicity by anti‐H‐2 b immune lymphocytes and absorption of anti‐H‐2 b antisera activity. The B16‐F1 line was able to efficiently kill allogeneic hosts, could not be lysed by anti‐H‐2 b cytotoxic effectors and did not express any serologically detectable amount of H‐2 b alloantigens. The B16‐A line was H‐2 positive during the early in vitro passages, then, at the 8th–10th passages, it acquired the capacity to kill allogeneic hosts, lost the sensitivity to anti‐H‐2 b cytotoxic effectors and the H‐2K b antigens became undetectable. The expression of H‐2D b was reduced, although at a lower degree. Similar data were obtained with B 16‐B cells, which after 10 in vitro passages grew and killed allogeneic hosts, showed a decreased sensitivity to cytotoxic anti‐H‐2 b effectors and a very low expression of the K region antigens. The results indicate that H‐2 expression is altered in B 16 melanoma lines and this may influence the different metastatic capacity of such cells.