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MOUSE MONOCLONAL ANTI‐N: I. PRODUCTION AND SEROLOGICAL CHARACTERIZATION
Author(s) -
Fraser R. H.,
Munro A. C.,
Williamson A. R.,
Barrie E. K.,
Hamilton E. A.,
Mitchell R.
Publication year - 1982
Publication title -
international journal of immunogenetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 47
eISSN - 1744-313X
pISSN - 1744-3121
DOI - 10.1111/j.1744-313x.1982.tb00985.x
Subject(s) - sialoglycoprotein , glycophorin , monoclonal antibody , epitope , sialic acid , antibody , sialoglycoproteins , serology , antigen , immunization , microbiology and biotechnology , biology , chemistry , virology , immunology , biochemistry
SUMMARY Two examples of mouse monoclonal anti‐N are described. The antibodies were derived from mice immunized with sialoglycoprotein extracts of group O MM ss erythrocyte membranes and the probable stimulus for immunization was glycophorin B associated N‐antigen. Both antibodies reacted as direct agglutinins but appeared to recognize different epitopes with one having a greater dependence on sialic acid. The antibodies could prove to be valuable alternatives to those reagents used currently for N‐blood grouping.