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Characterization of chitinase‐like proteins ( Cg ‐Clp1 and Cg ‐Clp2) involved in immune defence of the mollusc Crassostrea gigas
Author(s) -
Badariotti Fabien,
Lelong Christophe,
Dubos MariePierre,
Favrel Pascal
Publication year - 2007
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2007.05898.x
Subject(s) - chitinase , crassostrea , biology , oyster , gene , extracellular , cloning (programming) , extracellular matrix , lipopolysaccharide , microbiology and biotechnology , biochemistry , immunology , fishery , computer science , programming language
Chitinase‐like proteins have been identified in insects and mammals as nonenzymatic members of the glycoside hydrolase family 18. Recently, the first molluscan chitinase‐like protein, named Crassostrea gigas ( Cg )‐Clp1, was shown to control the proliferation and synthesis of extracellular matrix components of mammalian chondrocytes. However, the precise physiological roles of Cg ‐Clp1 in oysters remain unknown. Here, we report the cloning and the characterization of a new chitinase‐like protein ( Cg ‐Clp2) from the oyster Crassostrea gigas . Gene expression profiles monitored by quantitative RT‐PCR in adult tissues and through development support its involvement in tissue growth and remodelling. Both Cg ‐Clp1‐ and Cg ‐Clp2‐encoding genes were transcriptionally stimulated in haemocytes in response to bacterial lipopolysaccharide challenge, strongly suggesting that these two close paralogous genes play a role in oyster immunity.