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THE APPLICATION OF TECHNETIUM‐99m HEXAMETHYLPROPYLENEAMINE OXIME ( 99m Tc‐HMPAO) LABELED WHITE BLOOD CELLS FOR THE DIAGNOSIS OF RIGHT DORSAL ULCERATIVE COLITIS IN TWO HORSES
Author(s) -
East Leslie M.,
Trumble Troy N.,
Steyn Phillip F.,
Savage Catherine J.,
Dickinson Charles E.,
TraubDargatz Josie L.
Publication year - 2000
Publication title -
veterinary radiology and ultrasound
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.541
H-Index - 60
eISSN - 1740-8261
pISSN - 1058-8183
DOI - 10.1111/j.1740-8261.2000.tb02088.x
Subject(s) - medicine , ulcerative colitis , abdomen , horse , white blood cell , technetium 99m , dorsum , buffy coat , colitis , phenylbutazone , technetium , pathology , nuclear medicine , gastroenterology , scintigraphy , anatomy , surgery , paleontology , disease , biology
The application of 99m Tc‐HMPAO labeled white blood cells to support the diagnosis of right dorsal ulcerative colitis was studied in two horses with a history and clinical signs consistent with phenylbutazone toxicity. These images were compared to a reference horse unaffected by right dorsal ulcerative colitis. Blood was collected aseptically in heparinized syringes from the patients for in vitro white blood cell (WBC) radiolabeling. The buffy coat was separated out and radiolabeled with 99m TcHMPAO. The radiolabeled blood was re‐injected IV and four images of the right and left side of the patient's abdomen were acquired at 4 hours and 20 hours post‐injection. Results of the nuclear study revealed no abnormal findings in the abdomen at the four‐hour post‐injection images in any horse. Images obtained 20 hours post‐injection revealed a linear uptake of radiolabeled WBCs in the right cranioventral abdomen in the region of the right dorsal colon in both horses with right dorsal ulcerative colitis. The reference horse had no radiopharmaceutical uptake in this region. This nuclear imaging study was a rapid, non‐invasive method to identify right dorsal colon inflammation. These findings not only supported the diagnosis of right dorsal ulcerative colitis, but also facilitated appropriate medical management of each horse.