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Addition of erythrocytes to in vitro culture medium attenuates the detrimental effects of reactive oxygen species on bovine preimplantation embryo development
Author(s) -
IDETA Atsushi,
TSUCHIYA Kanami,
AOYAGI Yoshito
Publication year - 2012
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2011.00925.x
Subject(s) - blastocyst , hypoxanthine , embryo , in vitro , andrology , reactive oxygen species , embryogenesis , biology , in vitro maturation , embryo culture , xanthine oxidase , biochemistry , chemistry , microbiology and biotechnology , enzyme , medicine
ABSTRACT Erythrocytes were recently found to improve the early development of mice embryos by their antioxidant effect. The purpose of the present study was to examine the effect of erythrocytes on the in vitro development of bovine in vitro fertilized (IVF) embryos in medium supplemented with reactive oxygen species (ROS). IVF embryos were cultured in CR1aa medium supplemented with oxidizing agents, 0.5 mmol/L hypoxanthine and 0.01 U/mL xanthine oxidase (HX/XOD), in the presence and absence of erythrocytes (5 × 10 4 , 5 × 10 5 , 5 × 10 6 and 5 × 10 7 erythrocytes/mL). After 8 days, blastocysts were examined with a stereomicroscope. HX/XOD blocked development to the blastocyst stage (HX/XOD: 0%, control: 33%), but in the presence of both erythrocytes and HX/XOD, blastocyst development was restored to about one‐third to two‐thirds the normal rate (5 × 10 5 to 5 × 10 7 erythrocytes/mL: 12 to 23%). Furthermore, adding erythrocytes or erythrocyte hemolysate to medium without HX/XOD increased the blastocyst rate. These results suggest that the addition of erythrocytes can attenuate the detrimental effects of ROS on embryo development in bovine species as well as in mice.