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Ruminal distribution of the cellulolytic bacterium Fibrobacter succinogenes in relation to its phylogenetic grouping
Author(s) -
KOIKE Satoshi,
PAN Jun,
SUZUKI Tomoyuki,
TAKANO Toru,
OSHIMA Chihiro,
KOBAYASHI Yasuo,
TANAKA Keiichi
Publication year - 2004
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/j.1740-0929.2004.00207.x
Subject(s) - fibrobacter succinogenes , rumen , hay , biology , population , phylogenetic tree , straw , digestion (alchemy) , zoology , food science , agronomy , biochemistry , fermentation , chemistry , gene , demography , chromatography , sociology
ABSTRACT To investigate the ecological importance of the cellulolytic bacterium Fibrobacter succinogenes in fiber digestion, ruminal distribution of F. succinogenes was determined in relation to its phylogenetic grouping. Rumen digesta from wethers and steers fed orchardgrass hay, rice straw or fresh orchardgrass were employed as the materials for the analyses. Orchardgrass hay stem incubated in the rumen was also used. By using total DNA extracted from these materials, population sizes of total F. succinogenes and of four different phylogenetic groups of this species were quantitated through competitive polymerase chain reaction (PCR), and restriction fragment length polymorphism analysis of PCR products targeted the bacterial 16S rDNA. Rumen digesta and ruminally incubated hay stems had a reasonably high population size of F. succinogenes (×10 7−8 /g) that was composed of strains belonging to the phylogenetic groups 1 and 3. The relative abundance of each group was different among the samples; group 1 dominated on the ruminally incubated hay stem and in the rumen of wethers fed fresh orchardgrass, while group 3 was major in the rumen of wethers and steers on hay diet. These results suggest that there could be phenotypic differences among the phylogenetic groups of F. succinogenes , and group 1 dominating on hay stem might contribute to rumen fiber digestion more than the other groups.