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Immunohistochemical analysis of Fas ligand expression in normal human tissues
Author(s) -
Lee Sug Hyung,
Shin Min Sun,
Park Won Sang,
Kim Su Young,
Dong Seung Myung,
Lee Hun Kyung,
Park Jik Young,
Oh Ro Ra,
Jang Ja June,
Lee Jung Young,
Yoo Nam Jin
Publication year - 1999
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1999.tb01504.x
Subject(s) - fas ligand , biology , epithelium , immunohistochemistry , pathology , apoptosis , immunostaining , cell type , programmed cell death , cell , immunology , medicine , biochemistry , genetics
Cross‐linking of Fas and Fas ligand (FasL) induces apoptosis in Fas‐bearing cells and regulates apoptosis. Fas is widely expressed in normal human tissues, but FasL expression has been considered to be restricted to lymphoid tissues. Recent studies have demonstrated that FasL is also expressed in some nonlymphoid tissues. To screen the in situ expression of FasL in normal human tissues, immunohistochemistry was performed using paraffin‐embedded human tissues. FasL immunostaining was easily detected in testis, neurons, trophoblasts, tonsil, lymph node, Paneth cells, hepatocytes, renal tubular epithelium and bronchial epithelium, consistent with previous reports. Surprisingly, FasL was also expressed in many other cell types, including thymic medulla, skeletal muscle, cardiac muscle, pituitary gland, parathyroid gland, prostate glands, oocytes, epithelium of fallopian tube, endometrial glands, and gastric parietal cells. These findings demonstrate that FasL is widely expressed in human tissues and suggest that wide but cell‐type specific expression of FasL may not only be implicated in the regulation of immune homeostasis but also in the regulation of cell death and life in many cell types in vivo.