Premium
Estimation of type‐specific anti‐streptococcal M‐protein antibodies with biotinylated peptides in human sera
Author(s) -
KÜHNEMUND O.,
SCHUMACHER F.,
CASARETTO M.,
HOLM S. E.,
LÜTTICKEN R.
Publication year - 1998
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1998.tb01348.x
Subject(s) - biotinylation , antibody , specific antibody , microbiology and biotechnology , virology , biology , chemistry , immunology
The design of a novel enzyme‐linked immunosorbent assay for the estimation of antibodies directed against the N‐terminus of the M‐protein of Streptococcus pyogenes is described. The ELISA employs biotinylated peptide antigens of the types 1, 4, 12 and 19 immobilized by (strept‐)avidin on the surface of polystyrene microtiter wells. In rabbit hyperimmune sera and in human serum samples, antibodies against the corresponding serotype could be detected with high sensitivity and specificity.