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Lectin staining of renal tubules in normal kidney
Author(s) -
ENGEL ULLA,
BREBOROWICZ DANUTA,
BØGHANSEN THORKILD,
FRANCIS DORTHE
Publication year - 1997
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1997.tb00536.x
Subject(s) - jacalin , wheat germ agglutinin , lectin , concanavalin a , nephron , staining , peanut agglutinin , agglutinin , kidney , glycoprotein , chemistry , biology , microbiology and biotechnology , biochemistry , endocrinology , in vitro , genetics
Lectins are glycoproteins able to bind carbohydrate structures specifically. In this study we applied six different lectins on normal renal tissue to investigate their specificity for different segments of the renal tubular system. The following lectins were used: jacalin, peanut agglutinin (PNA), wheat germ agglutinin (WGA), phytohemagglutinin E (PHA‐E), concanavalin A (Con A), and Dolichos biflorus agglutinin (DBA). Particular attention was paid to jacalin lectin as its staining properties respecting the renal tubular system were not known. We showed that jacalin lectin strongly stains the luminal border of distal tubules, as well as single cells of the collecting tubules. As regards the other five lectins, PNA stained distal tubules, WGA the whole nephron, PHA‐E proximal tubules, and Con A and DBA a few cells of the loop of Henle.