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Two‐layer gradient separation of granulocytes for functional tests
Author(s) -
NæSs ARE,
Sjursen HAAKON,
Talstad INGEBRIGT,
Solberg CLAUS OLA
Publication year - 1990
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1990.tb05045.x
Subject(s) - separation (statistics) , chemotaxis , chromatography , dextran , separation method , granulocyte , chemistry , yield (engineering) , immunology , materials science , biology , mathematics , biochemistry , composite material , statistics , receptor
Two gradient separation techniques were compared with the dextran sedimentation method for the separation of granulocytes from blood. Both techniques gave adequate yield and excellent purity, and flow cytometric analysis of surface membrane markers gave no indication of subset selection during the procedure. Cells separated by the three methods behaved comparably in functional tests including random migration, chemotaxis and chemiluminescence. The gradient separation techniques are rapid and efficient methods for the preparation of near 100% pure granulocyte suspensions for functional studies.