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DNA content and nuclear size of megakaryocytes in thrombocythaemia
Author(s) -
Ridell B.,
Kutti J.,
Revesz P.,
BergstrÖM S.,
EnerbÄCk L.
Publication year - 1990
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1990.tb05005.x
Subject(s) - thrombocytosis , feulgen stain , platelet , nuclear dna , pathology , bone marrow , thrombopoiesis , biology , medicine , megakaryocyte , immunology , staining , haematopoiesis , genetics , stem cell , gene , mitochondrial dna
Total nuclear DNA content and nuclear size of megakaryocytes were studied in biopsies of the iliac bone marrow of individuals with normal or increased platelet counts. The DNA content was determined using Feulgen cytophotometry of bone marrow smears and the nuclear area by morphometric analysis of megakaryocytes of bone marrow sections. The mean DNA content and the mean nuclear area were both significantly larger in megakaryocytes of patients with thrombocytosis as a result of myeloproliferative disease than in patients with secondary thrombocytosis as well as in two control groups of individuals with normal platelets counts, one comprising healthy volunteers, the other with various non‐haematological disorders. There was a statistically significant correlation between the DNA content and nuclear area of the megakaryocytes (r = 0,92) in the entire group of bone marrows studied.