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PURIFICATION OF ANTI‐LACTOSE ANTIBODIES FROM ANTISERA TO NEISSERIA GONORRHOEAE AND NEISSERIA MENINGITIDIS
Author(s) -
Brakstad Odd Gunnar,
Mæland Johan A.
Publication year - 1985
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1985.tb02936.x
Subject(s) - neisseria gonorrhoeae , neisseria meningitidis , antiserum , antibody , microbiology and biotechnology , lactose , lipopolysaccharide , neisseriaceae , affinity chromatography , sepharose , serotype , neisseria , chemistry , bacteria , biology , antibiotics , enzyme , biochemistry , immunology , genetics
Lactosyl‐sepharose 4B columns were used for purification by affinity chromatography of anti‐lactose antibodies from rabbit antisera to the gonococcal strains 8551 and VII, and the serogroup B meningococcal strain M982. Anti‐lactose antibodies were obtained from all three antisera. SDS‐PAGE of lipopolysaccharide and of bacterial cells and immunoblotting with the anti‐lactose antibodies showed that the lipopolysaccharide was the only bacterial component with binding sites for the antibodies.