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EFFECTS OF METOTREXATE, AMPICILLIN AND GENTAMICIN ALONE AND IN COMBINATION ON THE IN VITRO LOCOMOTION ON HUMAN POLYMORPHONUCLEAR CELLS (PMN)
Author(s) -
Melby Kjetil,
Quie Paul G.
Publication year - 1984
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1984.tb00096.x
Subject(s) - gentamicin , ampicillin , zymosan , antibiotics , in vitro , chemistry , microbiology and biotechnology , pharmacology , biology , biochemistry
The effect of ampicillin 25 mg/ml, gentamicin 5 mg/1 and metotrexate 10 & 70 mg/I, alone and in combination, was tested for their influence on human polymorphonuclear cell‐locomotion, using zymo‐san or a bacterial filtrate of E.coli, with or without 0.25 and 0.025 mg/1 of ampicillin and gentamicin, respectively, as attractants. Metotrexate and gentamicin alone decreased the locomotion, using zymosan as attractant. No effect was observed using the pure bacterial filtrate. The filtrate containing antibiotics displayed a lower ability to induce locomotion when the leucocytes had been pre‐treated with antibiotics alone or in combination with metotrexate but not with metotrexate alone. Aspects of these findings are discussed.