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QUANTITATION OF PROENZYME Clr‐Cls COMPLEXES IN ERUM BY ELECTROIMMUNOASSAY
Author(s) -
Laurell A. B.,
Mârtensson U.,
Sjöholm A. G.
Publication year - 1981
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1981.tb02718.x
Subject(s) - cls upper limits , chemistry , electrophoresis , immunoelectrophoresis , biochemistry , antibody , medicine , immunology , optometry
The electroimmunoassay for Clr‐Cls complexes was performed in two electrophoretic steps, the first in the presence of Ca2+ without antibody, and the second in the presence of EDTA with anti‐Clr in the gel. The results were reproducible and in agreement with semiquantitative estimates of Clr‐ClS complexes in normal and pathological sera on crossed immunoelectrophoresis.