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LOCALIZATION OF THE C3b RECEPTORS IN HUMAN RENAL GLOMERULI
Author(s) -
MATRE ROALD,
NILSEN RLNE,
IVERSEN BJARNE M.
Publication year - 1981
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1981.tb02705.x
Subject(s) - horseradish peroxidase , receptor , peroxidase , chemistry , kidney , guinea pig , electron microscope , microbiology and biotechnology , biology , biochemistry , enzyme , endocrinology , physics , optics
Cryostat sections of human kidney were incubated with C3b containing immune complexes. The complexes were horseradish peroxidase (HRP) and Flab'b fragments of rabbit IgG anii‐HRP incubated in C4‐deficient guinea pig serum. The peroxidase activity was revealed histochemically. The sections were studied using light and electron microscopy. The bound complexes were localized to the foot processes of the visceral epithelial cells and to the surface of the parietal epithelial cells facing Bowman's space. No receptors could be demonstrated on the endothelial or mesangial cells.