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A MORPHOMETRICAL METHOD FOR ANALYZING GERMINAL CENTERS IN THE CHICKEN SPLEEN
Author(s) -
ROMPPANEN TIMO
Publication year - 1981
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1981.tb02697.x
Subject(s) - germinal center , spleen , biology , anatomy , immunology , antibody , b cell
A morphometric method for the analysis of the germinal centers in the chicken spleen is presented. Based on counts in the two‐dimensional tissue sections, the following three‐dimensional germinal center characteristics were calculated: volume density (Vvgc) numerical density (Nvgc); total number in the spleen (NgCsp); nearest neighbour distance (δ 3 ), and mean diameter (D). The total number of the germinal centers in the spleen of the six‐week‐old and the ten‐week‐old unimmunized chickens was 803 and 642, respectively. The mean diameter of the germinal centers was 102–108 pm, but its size distribution ranged from 50 to 175 μm.