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OCHRATOXIN A‐INDUCED MYCOTOXIC PORCINE NEPHROPATHY: ALTERATIONS IN ENZYME ACTIVITY IN TUBULAR CELLS
Author(s) -
ELLING FOLMER
Publication year - 1979
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1979.tb00048.x
Subject(s) - lactate dehydrogenase , dehydrogenase , succinate dehydrogenase , alkaline phosphatase , enzyme , biochemistry , isocitrate dehydrogenase , chemistry , medicine , acid phosphatase , ochratoxin a , endocrinology , biology , food science , mycotoxin
Mycotoxic porcine nephropathy was induced by p.o. administration of crystalline ochratoxin A for periods of 5 days, 3 months and 2 years. Enzyme activities of the renal tissue were studied histochemically. These were NADH‐tetrazolium reductase, NADPH‐tetrazolium reductase, lactate dehydrogenase, isocitrate dehydrogenase, succinate dehydrogenase, glucose‐6‐phosphate dehydrogenase, α‐glycerophosphate dehydrogenase, unspecific acid phosphatase and unspecific alkaline phosphatase. The activity of NADH‐tetrazolium reductase and succinate dehydrogenase was reduced in the proximal tubule of all nephrons after 5 days ochratoxin A exposure and remained reduced after 3 months and 2 years exposure. The effect of ochratoxin A on these enzymes would appear to cause the impairment of proximal tubular function and the morphological changes observed in the proximal tubule in ochratoxin A‐induced mycotoxic porcine nephropathy. The localization of alterations in enzyme activity corresponds to the localization of ochratoxin A previously demonstrated in the kidney. The activities of NADPH‐tetrazolium reductase, lactate dehydrogenase, glucose‐6‐phosphate dehydrogenase and unspecific alkaline phosphatase were reduced focally corresponding to the areas with focal tubular atrophy and the degree of reduction was roughly parallel to the degree of atrophy.