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A RADIOIMMUNOASSAY FOR OCHRATOXIN A: A PRELIMINARY INVESTIGATION
Author(s) -
Aalund O.,
Brunfeldt K.,
Hald B.,
Krogh P.,
Poulsen K.
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1975.tb01655.x
Subject(s) - ochratoxin a , radioimmunoassay , ochratoxin , metabolite , chemistry , chromatography , antigen , aspergillus , bovine serum albumin , biochemistry , mycotoxin , food science , biology , microbiology and biotechnology , immunology
Ochratoxin A is a nephrotoxic secondary metabolite of several fungal species included in the genera Aspergillus and Penicillium . A radioimmunoassay for this toxin is described. The reagents included antibodies against a coupling product of ochratoxin A and bovine IgG. 125 I‐labelled egg‐albumin‐ochratoxin A was employed as radioactive antigen. Free ochratoxin A dissolved in phosphate‐buffered saline inhibited immunoprecipitation of the radioactive antigen. The procedure showed a lower limit of detection at 20 ppb of ochratoxin A.