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Rabbit Anti Rat Lymphocyte Serum: Immunosuppression Mediated by IgM Antibodies
Author(s) -
Jakobsen Arnt,
Flatmark Audun
Publication year - 1972
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1972.tb00172.x
Subject(s) - antiserum , azathioprine , spleen , immunosuppression , cytotoxic t cell , antibody , immunization , lymphocyte , immunology , lymph , titer , biology , medicine , pathology , in vitro , biochemistry , disease
Rabbit anti rat lymphocyte sera (ALS) were produced in rabbits treated with azathioprine during the immunization period. Such antisera were cytotoxic to lymphocytes with nearly as high litres as have been seen in untreated animals using the same immunization schedule. Treatment of the antisera with 2‐mercaptoethanol followed by alkylation, resulted in marked loss in titre towards lymphocytes. Separation on Sephadex G‐200 confirmed that most of the cytotoxic activity was to be found among the IgM antibodies. Both “thymus‐azathioprine” and “spleen‐azathioprine” ALS significantly prolonged the survival of skin allografts in rats. Thymus antisera were more efficient than spleen antisera. Reduction and alkylation of active thymus antisera resulted in loss of in vivo immunosuppressive effect.
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