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IN VITRO STUDIES ON THE FATE OF ANTIGEN
Author(s) -
Rhodes J. M.
Publication year - 1971
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1971.tb02142.x
Subject(s) - ferritin , in vitro , immunoelectrophoresis , spleen , incubation , antigen , chemistry , in vivo , albumin , human serum albumin , biochemistry , microbiology and biotechnology , serum albumin , digestion (alchemy) , biology , chromatography , immunology
Extracts of mouse spleen and mouse peritoneal macrophages digested human serum albumin (HSA) at pH 3.0 in vitro ; the products of digestion gave three different precipitating components as shown by immunoelectrophoresis. The extent to which 125 I‐HSA was degraded could also be followed by assaying the radioactivity on a paper strip after zone electrophoresis of the HSA digests. The presence of ferritin did not affect the digestion of HSA. Ferritin was not cleaved by the spleen or cell extract at pH 3.0, 4.5 or 8.0, even after incubation at 37d̀C for 24 hours. These results are discussed in relation to the fate of the antigens in vivo.