Open AccessRibosomal DNA intergenic spacer sequence in foxtail millet, Setaria italica (L.) P. Beauv. and its characterization and application to typing of foxtail millet landraces.
Author(s) -
FUKUNAGA KENJI,
ICHITANI KATSUYUKI,
TAURA SATORU,
SATO MUNEHARU,
KAWASE MAKOTO
Publication year - 2005
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/j.1601-5223.2005.01905.x
Subject(s) - foxtail , biology , setaria , ribosomal dna , intergenic region , ribosomal rna , botany , ribosomal intergenic spacer analysis , genetics , gene , phylogenetics , genome
We determined the sequence of ribosomal DNA (rDNA) intergenic spacer (IGS) of foxtail millet isolated in our previous study, and identified subrepeats in the polymorphic region. We also developed a PCR‐based method for identifying rDNA types based on sequence information and assessed 153 accessions of foxtail millet. Results were congruent with our previous works. This study provides new findings regarding the geographical distribution of rDNA variants. This new method facilitates analyses of numerous foxtail millet accessions. It is helpful for typing of foxtail millet germplasms and elucidating the evolution of this millet.