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Cytotoxicity of three resin‐based root canal sealers: an in vitro evaluation
Author(s) -
Koulaouzidou E. A.,
Papazisis K. .,
Beltes P.,
Geromicholos G. D.,
Kortsarls A. H.
Publication year - 1998
Publication title -
dental traumatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 81
eISSN - 1600-9657
pISSN - 1600-4469
DOI - 10.1111/j.1600-9657.1998.tb00834.x
Subject(s) - hemocytometer , cytotoxicity , root canal , in vitro , chemistry , pulp (tooth) , dentistry , medicine , biochemistry
— The cytotoxicity of three resin‐based root canal sealers (AH26, AH‐Plus, Topseal) was evaluated in vitro. The experiments included two cell lines, L929 mouse skin fibroblasts and RPC‐C2A rat pulp cells. The cytotoxicity was assessed by sulforodamine B (SRB) colorimetric assay and hemocytometer viable cell counting after 24‐ and 48‐h exposure. AH26 had a severe cytotoxic effect whilst Topseal and AH‐Plus showed a markedly lower toxic influence on the cells during the experimental period.

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