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Demonstration of Bacteroides intermedius in periapical tissue using indirect immunofluorescence microscopy
Author(s) -
Barnett Frederic,
Stevens Roy,
Tronstad Leif
Publication year - 1990
Publication title -
dental traumatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 81
eISSN - 1600-9657
pISSN - 1600-4469
DOI - 10.1111/j.1600-9657.1990.tb00411.x
Subject(s) - streptococcus intermedius , pathology , immunofluorescence , bacteroides , pulp (tooth) , granuloma , staining , antiserum , direct fluorescent antibody , biology , antibody , medicine , bacteria , streptococcus , immunology , genetics
The presence of bacteria in periapical lesions of teeth with necrotic pulp has been demonstrated by microbiological sampling of periapical lesions during endodontic surgery. The purpose of this study was to confirm the presence of Bacteroides intermedius in a periapical granuloma using an indirect immunofluorescence technique. Histologic sections of a periapical granuloma were incubated with a rabbit antiserum to B. intermedius. After incubation with secondary antibody (fluorescein‐conjugated goat anti‐rabbit IgG), an intense fluorescent staining was observed in areas of the tissue sections. Control tissue sections that were incubated without the antibody remained unstained. The results obtained with the indirect immunofluorescence technique supported our cultural findings that microorganisms, e.g. B. intermedius, were present in the tissue of the periapical granuloma.