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ORIGINAL ARTICLE: H3N2 Influenza A Virus Replicates in Immortalized Human First Trimester Trophoblast Cell Lines and Induces Their Rapid Apoptosis
Author(s) -
Trinh Quang Duy,
Izumi Yasuyuki,
KomineAizawa Shihoko,
Shibata Toshikatsu,
Shimotai Yoshitaka,
Kuroda Kazumichi,
Mizuguchi Masashi,
Ushijima Hiroshi,
Mor Gil,
Hayakawa Satoshi
Publication year - 2009
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2009.00723.x
Subject(s) - trophoblast , biology , virus , apoptosis , virology , human leukocyte antigen , influenza a virus , immunology , viral replication , cell culture , antigen , pregnancy , fetus , placenta , genetics
Problem Epidemiological data suggested that pandemic influenza increased the risks of spontaneous abortion and premature labor, while seasonal influenza also increased the risk of schizophrenia in adolescence. However, their pathogenesis is so far unknown. Method of study The first trimester trophoblast cell lines, namely, Swan71 and HTR8 cells were challenged with A/Udorn/72 influenza virus (H3N2). At indicated time points, cells were examined for expression of influenza proteins. Viral replication in culture media, apoptosis and the expression of human leukocyte antigen (HLA)‐G were also examined. Results Intracellular localization of viral proteins was observed. Twenty‐four hours after inoculation, virus was detected in culture media while most cells fell into apoptosis. During apoptosis, expression of HLA‐G was unchanged. Conclusion We revealed replication of low pathogenic influenza virus in the first trimester trophoblast cell lines. Placental damages are likely to be induced by direct cytopathic effects of influenza virus and subsequent apoptosis rather than down regulation of HLA‐G expression and subsequent rejection by maternal immune system.