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Presence of Leukemia Inhibitory Factor and Interleukin‐12 in Human Follicular Fluid During Follicular Growth
Author(s) -
Coskun Serdar,
Uzumcu Mehmet,
Jaroudi Kamal,
Hollanders Johannes M.G.,
Parhar Ranjit S.,
AlSedairy Sultan T.
Publication year - 1998
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1998.tb00382.x
Subject(s) - follicular fluid , leukemia inhibitory factor , follicular phase , medicine , endocrinology , human chorionic gonadotropin , chemistry , ovarian follicle , andrology , in vitro , biology , cytokine , interleukin 6 , oocyte , hormone , microbiology and biotechnology , biochemistry , embryo
PROBLEM: Cytokines have been shown to be present in human follicular fluid and have regulatory functions on follicular maturation. The presence of leukemia inhibitory factor (LIF) and interleukin (IL)‐12 in human follicular fluid obtained at different stages of maturation was investigated. METHOD OF STUDY: Follicular fluids and granulosa cells were obtained from preovulatory and immature follicles. Follicular fluids from both groups were assayed for IL‐12 and LIF by enzyme‐linked immunosorbent assay. Granulosa cells from preovulatory and immature follicles were treated with human chorionic gonadotropin (hCG) in vitro and subsequent LIF and IL‐12 production were measured. RESULTS: The average concentration of LIF was significantly higher in preovulatory follicles (7.6 ± 1.3 pg/ml, n = 24) than in immature follicles (2.0 ± 1.3 pg/ml, n = 6). The concentration of IL‐12 was significantly higher in follicular fluid obtained from immature follicles (10.9 ± 5.0 pg/ml) than in preovulatory follicles (1.3 ± 0.4 pg/ml). hCG only stimulated LIF production from mature granulosa cells; it had no effect on IL‐12 production. CONCLUSIONS: IL‐12 and LIF are present in follicular fluid and their levels are regulated differently during follicular maturation. hCG stimulates LIF production from granulosa cells in vitro.