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Detection of Immunoregulatory Lipid‐Like Factors in Human Amniotic Fluid
Author(s) -
RUEDA RICARDO,
VARGAS MA LUISA,
GARCÍAPACHECO MARCOS,
GARCÍAOLIVARES ENRIQUE
Publication year - 1990
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.1990.tb01036.x
Subject(s) - amniotic fluid , chemistry , conceptus , immune system , immunology , biology , fetus , pregnancy , genetics
The immunosuppressive activity of amniotic fluid (AF) is extensively documented in the mouse. Although this property is due in part to the presence of alpha‐fetoprotein (α‐FP), other immunosuppressive factors are suspected. In this article, we demonstrate that human amniotic fluid lipid extract (AFLE) is inhibitory of, although not cytotoxic to, PHA‐activated human lymphocytes, of mouse bone marrow cells, and of different established cell lines of human and mouse source. This effect is shown to be reversible. Under preparative thin layer chromatography (TLC) using chloroform:methanol:water (60:38:8) as solvents, the activity of AFLE migrates to two peaks of inhibition with Rf values of 0.46‐0.62 and 0.84‐1, respectively. These lipid‐like factors may play a role as a nonspecific immunoregulatory mechanism which prevents maternally mediated immune rejection of the conceptus.