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Monoclonal Antibody Inducing Human Sperm Agglutination
Author(s) -
YAN YUAN CHANG,
WANG LIN FANG,
SATO EIMEI,
KOIDE S.S.
Publication year - 1983
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
eISSN - 1600-0897
pISSN - 0271-7352
DOI - 10.1111/j.1600-0897.1983.tb00263.x
Subject(s) - immunofluorescence , sperm , monoclonal antibody , agglutination (biology) , antibody , antigen , western blot , biology , microbiology and biotechnology , indirect immunofluorescence , immunology , biochemistry , genetics , gene
A mouse fused splenic cell line producing antibody that induces agglutination of human sperm was cultivated. The antibody was localized to the midpiece and tail of human spermatozoa by an indirect immunofluorescence method. The interacting antigen was identified in a sperm membrane protein preparation by SDS gel/protein blot immunofluorescence method. The molecular weight of the antigen was in the vicinity of 84 Kdaltons.