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Rab6‐interacting Protein 1 Links Rab6 and Rab11 Function
Author(s) -
MisereyLenkei Stéphanie,
Waharte François,
Boulet Annick,
Cuif MarieHélène,
Tenza Danielle,
El Marjou Amed,
Raposo Graça,
Salamero Jean,
Héliot Laurent,
Goud Bruno,
Monier Solange
Publication year - 2007
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/j.1600-0854.2007.00612.x
Subject(s) - endosome , cytokinesis , microbiology and biotechnology , biology , golgi apparatus , mitosis , rab , bimolecular fluorescence complementation , transport protein , biochemistry , cell , cell division , intracellular , gtpase , endoplasmic reticulum , yeast
Rab11 and Rab6 guanosine triphosphatases are associated with membranes of the recycling endosomes (REs) and Golgi complex, respectively. Evidence indicates that they sequentially regulate a retrograde transport pathway between these two compartments, suggesting the existence of proteins that must co‐ordinate their functions. Here, we report the characterization of two isoforms of a protein, Rab6‐interacting protein 1 (R6IP1), originally identified as a Rab6‐binding protein. R6IP1 also binds to Rab11A in its GTP‐bound conformation. In interphase cells, R6IP1 is targeted to the Golgi in a Rab6‐dependent manner but can associate with Rab11‐positive compartments when the level of Rab11A is increased within the cells. Fluorescence resonance energy transfer analysis using fluorescence lifetime imaging shows that the overexpression of R6IP1 promotes an interaction between Rab11A and Rab6 in living cells. Accordingly, the REs marked by Rab11 and transferrin receptor are depleted from the cell periphery and accumulate in the pericentriolar area. However, endosomal and Golgi membranes do not appear to fuse with each other. We also show that R6IP1 function is required during metaphase and cytokinesis, two mitotic steps in which a role of Rab6 and Rab11 has been previously documented. We propose that R6IP1 may couple Rab6 and Rab11 function throughout the cell cycle.