Premium
Improved Method for the Measurement of Chicken and Rat Pineal Serotonin N‐Acetyltransferase Activity
Author(s) -
RodriguezCabello J. C.,
Arpa F.,
Agapito M. T.,
GarciaHerrero I.,
Recio J. M.
Publication year - 1990
Publication title -
journal of pineal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 131
eISSN - 1600-079X
pISSN - 0742-3098
DOI - 10.1111/j.1600-079x.1990.tb00698.x
Subject(s) - egta , serotonin , melatonin , homogenization (climate) , divalent , incubation , endocrinology , biochemistry , medicine , chemistry , biology , calcium , receptor , biodiversity , ecology , organic chemistry
EGTA, a nonspecific ion chelator, showed very good potential for preventing the loss of serotonin N‐acetyl transferase activity (arylamine: acetyl‐CoA: N‐acetyltransferase, EC 2. 3. 1. 5.) (SNAT) from rat and chicken pineal glands during preincubation at 37°C before substrate addition. SNAT activity was higher than that of the controls when EGTA was present in the homogenization buffer due to the prevention of the loss of activity probably not only during the preincubation period but also during incubation with its substrates. This characteristic of EGTA suggests that its use is suitable in the improved SNAT assay described in this paper since higher SNAT activities were found in both rat and chicken pineal glands than the activities recorded with the usual methods.