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Effect of periodontal therapy on salivary enzymatic activity
Author(s) -
Zambon Joseph J.,
Nakamura Masakazu,
Slots Jorgen
Publication year - 1985
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1985.tb00850.x
Subject(s) - saliva , bacteroides , gingival and periodontal pocket , prevotella intermedia , microbiology and biotechnology , medicine , dentistry , chemistry , periodontitis , porphyromonas gingivalis , biology , bacteria , genetics
This study determined changes in the levels of enzymes in whole mixed saliva of adults following periodontal therapy including two or more sessions of meticulous periodontal scaling and root planing and 1 g/day of tetracycline‐HCI for 14 days. Clinical variables assessed at baseline and 2 weeks after treatment included Gingival Index, Plaque Index, periodontal pocket depth, and gingival crevicular fluid flow. Subgingival specimens obtained from the deepest periodontal pocket in each quadrant were analyzed for Bacteroides gingivalis and Bacteroides intermedius using indirect immunofluorescence microscopy and for bacterial morphotypes using bright light phase contrast microscopy. The enzymatic profile of unstimulated whole mixed saliva was determined using the API ZYM and API ZYM AP micromethod systems. Periodontal treatment significantly reduced gingival inflammation and the proportions of subgingival black‐pigmented bacteroides and motile organisms. However, the Plaque Index and total salivary bacterial counts remained virtually unchanged. Treatment also resulted in reduced salivary levels of caprylate esteraselipase, leucine, valine, and cystine aminopeptidases, trypsin, β‐galactosidase, β‐glucuronidase, and β‐glucosidase. The present study indicates that the efficacy of periodontal treatment may be readily monitored by changes in levels of activity of specific enzymes in whole saliva.