Premium
A freeze‐fracture study of the periodontal ligament of the rat
Author(s) -
Cho MoonIl,
Garant Philias R.
Publication year - 1983
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1983.tb00359.x
Subject(s) - periodontal fiber , fibroblast , membrane , ultrastructure , cytoplasm , protoplasm , organelle , extracellular matrix , cell , microbiology and biotechnology , matrix (chemical analysis) , golgi apparatus , ligament , cell membrane , fracture (geology) , chemistry , biophysics , materials science , anatomy , biology , dentistry , composite material , biochemistry , medicine , in vitro
Fibroblasts of the rat periodontal ligament were studied in situ by the freeze‐fracture technique. Large surfaces of the protoplasmic and extracellular faces of the cell membrane as well as, the membranes of the RER, Golgi apparatus, and other cytoplasmic organelles were viewed at the ultrastructural level. Intramembrane particles representing membrane proteins were visualized, and their concentration in various membranes measured. Features of the fibroblast cell surface appearing as rough patches were interpreted to represent areas of possible cell‐to‐matrix interaction. The use of the freeze‐fracture technique in further studies of PDL fibroblast‐matrix contact is discussed.