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A method for the quantitation of the epithelium‐connective tissue interface of the oral mucosa
Author(s) -
KARRING T.,
LÖE HARALD
Publication year - 1971
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1971.tb02016.x
Subject(s) - connective tissue , epithelium , basement membrane , pathology , anatomy , oral mucosa , chemistry , biology , medicine
– A method has been developed for quantitating, on the basis of histologic serial sections, the contact area between the epithelium and connective tissue of oral mucosa. Magnified (200 ×) paper replicas are produced from histologic sections (20 microns) and two consecutive paper replicas are superimposed on an illuminated grid. By means of the grid lines the area between the two basement membranes is divided into trapezoids (T 1 ) which represent the projection of imaginary trapezoids (T 2 ) connecting the basement membranes of two adjacent sections. The area of these T 2 ‐trapezoids may be calculated and is considered as representative for the epithelium‐connective tissue interface. Depending on the appearance of the T 1 ‐trapezoids, a certain amount of discrepancy occurs between the calculated and actual area of the T 2 ‐trapezoids. The significance of this is examined in thirty gingival specimens. It is concluded that without producing accurate values, the method offers the possibility to study quantitative differences in the epithelium‐connective tissue interface.