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H‐ras‐1 restriction fragment length polymorphism in normal individuals and oral cancer patients in India
Author(s) -
Bhoite Leena T.,
Saranath Dhananjaya,
Nair Rama,
Deo Madhav G.,
Sanghavi Vikram,
Mehta Ashok
Publication year - 1993
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1993.tb01076.x
Subject(s) - allele , restriction fragment length polymorphism , genotype , locus (genetics) , genetics , biology , allele frequency , microbiology and biotechnology , gene
Restriction fragment length polymorphism (RFLP) of the human H‐ras‐l gene has been indicated as a marker for detection of individuals at high risk of cancer. We have investigated the association of RFLP at the H‐ras‐l locus and susceptibility to oral cancer by Southern hybridization analysis in 77 primary oral tumors and 99 healthy donors. The frequency distribution of the Bum HI fragments of H‐ras‐l revealed homozygous or heterozygous alleles in the two sub‐populations. The heterozygous genotype occurred more frequently in the normal subjects (53%) as compared to the cancer patients (36%). Four common alleles Cl to C4, were noted predominantly in both groups, with rare alleles detected at a lower frequency. The common allele with 7.6 kb BamHI fragment was significantly higher in normals (10%) than in the tumor population (4%) ( P ≤0.05). However, a similar distribution of rare alleles in both groups indicated that the presence of rare alleles is not indicative of predisposition to oral cancer.