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Activity levels of some oxidoreductases of carbohydrate metabolism in leukoplakic and normal human buccal epithelium
Author(s) -
Gerson Stanley,
Silverman Sol
Publication year - 1973
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/j.1600-0714.1973.tb01843.x
Subject(s) - epithelium , dehydrogenase , glyceraldehyde 3 phosphate dehydrogenase , pentose phosphate pathway , isocitrate dehydrogenase , biology , biochemistry , nad+ kinase , glyceraldehyde , enzyme , chemistry , glycolysis , microbiology and biotechnology , genetics
Abstract. The levels of activity of three oxidoreductases (glyceraldehyde‐3‐phosphate, NAD‐de‐pendent isocitrate, and glueose‐6‐phosphate dehydrogenases) were determined in pathologically keratinized (leukoplakic) and adjacent normal nonkeratinzed epithelium from the buccal mucosa of humans. Samples of histologically identifiable epithelial layers as well as of whole epithelium were isolated by microdissection from frozen‐dried sections and assayed by ultramicrochemical methods. In the leukoplakic epithelium, as compared to the normal, glyceraldehyde‐3‐phosphate dehydrogenase activity was slightly higher, NAD‐dependent isocitrate dehydrogenase activity was more than twice as high, and glucose‐6‐phosphate dehydrogenase activity was about l/3 lower. These findings suggest a higher potential for the formation of energy‐rich compounds but a lower potential for pentose phosphogluconate pathway activity in the leukoplakic epithelium.