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A novel xenograft model of cutaneous T‐cell lymphoma
Author(s) -
Krejsgaard Thorbjørn,
Kopp Katharina,
Ralfkiaer Elisabeth,
Willumsgaard Ayelah E.,
Eriksen Karsten W.,
Labuda Tord,
Rasmussen Susanne,
Mathiesen AnneMerete,
Geisler Carsten,
Lauenborg Britt,
Becker Jürgen C.,
Zhang Qian,
Wasik Mariusz A.,
Odum Niels,
Woetmann Anders
Publication year - 2010
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2010.01138.x
Subject(s) - medicine , pathology , cutaneous t cell lymphoma , lymphoma , lymphatic system , transplantation , t cell , lymph , cancer research , nod , mycosis fungoides , immunology , immune system , diabetes mellitus , endocrinology
Please cite this paper as: A novel xenograft model of cutaneous T‐cell lymphoma. Experimental Dermatology 2010; 19 : 1096–1102. Abstract: Cutaneous T‐cell lymphomas (CTCLs) are characterized by accumulation of malignant T cells in the skin. Early disease resembles benign skin disorders but during disease progression cutaneous tumors develop, and eventually the malignant T cells can spread to lymph nodes and internal organs. However, because of the lack of suitable animal models, little is known about the mechanisms driving CTCL development and progression in vivo . Here, we describe a novel xenograft model of tumor stage CTCL, where malignant T cells (MyLa2059) are transplanted to NOD/SCID‐B2m −/− (NOD.Cg‐Prkdc scid B2m tm1Unc /J) mice. Subcutaneous transplantation of the malignant T cells led to rapid tumor formation in 43 of 48 transplantations, whereas transplantation of non‐malignant T cells isolated from the same donor did not result in tumor development. Importantly, the tumor growth was significantly suppressed in mice treated with vorinostat when compared to mice treated with vehicle. Furthermore, in most mice the tumors displayed subcutaneous and/or lymphatic dissemination. Histological, immunohistochemical and flow cytometric analyses confirmed that both tumors at the inoculation site, as well as distant subcutaneous and lymphatic tumors, originated from the transplanted malignant T cells. In conclusion, we describe a novel mouse model of tumor stage CTCL for future studies of disease dissemination and preclinical evaluations of new therapeutic strategies.