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Pro‐inflammatory cytokine release in keratinocytes is mediated through the MAPK signal‐integrating kinases
Author(s) -
Kjellerup Rasmus Boye,
Kragballe Knud,
Iversen Lars,
Johansen Claus
Publication year - 2008
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2007.00672.x
Subject(s) - mapk/erk pathway , kinase , cytokine , extracellular signal regulated kinases , signal transduction , microbiology and biotechnology , medicine , cancer research , chemistry , immunology , biology
Abstract: The mitogen‐activated protein kinases (MAPKs) are known to play a key role in the regulation of cytokine expression in several cell types. MAPK signal‐integrating kinase 1 (Mnk1) is a kinase activated through both the stress‐ and cytokine‐activated p38 MAPK pathway and the classical extracellular signal‐regulated kinase 1/2 (ERK1/2) pathway. In this study, we demonstrate that in cultured normal human keratinocytes Mnk1 and its downstream target eukaryotic initiation factor 4E (eIF4E) are phosphorylated in a time‐dependent manner in response to stimulation with anisomycin or interleukin (IL)‐1β. Both the stimuli are well‐recognized activators of the p38 MAPK pathway. Furthermore, we show that the Mnk inhibitor CGP57380 is capable of inhibiting the phosphorylation of eIF4E in keratinocytes, and that the abolishment of eIF4E phosphorylation dramatically decreases the anisomycin‐induced protein release of the pro‐inflammatory cytokines tumor necrosis factor‐α (TNF‐α), IL‐1β and IL‐6 as well as the IL‐1β‐induced protein release of TNF‐α. Therefore, we propose that Mnk1 might contribute to the expression of pro‐inflammatory cytokines found in inflammatory skin diseases.