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Detection of Normal and Malignant Megakaryocytes by Anti β‐Thromboglobulin Serum
Author(s) -
Pizzolo G.,
Chilosi M.,
Schiavon R.,
Ambrosetti A.,
Cetto G. L.,
Sabbioni R.,
Caramaschi G.,
Perona G.
Publication year - 1982
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1982.tb00583.x
Subject(s) - antiserum , megakaryocyte , platelet , pathology , bone marrow , immunofluorescence , peripheral blood , medicine , malignant cells , immunology , biology , antibody , haematopoiesis , cancer , stem cell , genetics
An antiserum to β‐thromboglobulin was used in immunofluorescence to detect normal and malignant megakaryocytes. In normal peripheral blood and bone marrow smears, only platelets and megakaryocytes were specifically stained by this antiserum. Among 25 cases of acute lymphoid and non‐lymphoid leukaemia, only 2 exhibited a clear positivity in a % of blasts, thus proving their megakaryocytic origin. This method is proposed as a simple and useful tool to detect normal and malignant megakaryocytes on smears.