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Proliferation of Human Lymphoid Cells Lymphocytopoiesis and Cell Cycle Parameters in Isolated Perfused Human Spleens
Author(s) -
Pabst R.,
Reinecke G.
Publication year - 1981
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1981.tb01622.x
Subject(s) - spleen , lymphatic system , mitotic index , thymidine , labelling , mitosis , hyperplasia , lymphoid hyperplasia , biology , pathology , perfusion , chemistry , microbiology and biotechnology , andrology , immunology , lymphoma , in vitro , endocrinology , medicine , biochemistry
Parameters of the cell cycle of lymphoid cells and the rate of production of small lymphocytes and mature plasma cells were estimated in human spleens. 12 normal and 1 enlarged spleen with lymphatic hyperplasia were labelled either by a pulse or continuously with 3 H‐TdR‐thymidine during normothermic perfusion of the isolated spleens. Biopsies of splenic tissue were taken at different intervals and evaluated autoradiographically. The mean initial labelling index of immunoblasts was 73% and for plasmoblasts 52%. The labelling index for small lymphocytes and mature plasma cells increased initially from 0 % to 1 %, and up to 25 % respectively after 14 h. The duration of the S‐phase, as determined by double labelling, was 8.26 h for lymphoid blasts. Data from the percentage labelled mitosis curve resulted in a minimal t G2 of about 1 h and t G2 + t M of about 4 h. The estimated generation time was about 11.5 h. The results found on the hyperplastic spleen were comparable to those on the normal spleen.