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Deoxyuridine‐Suppression Test and Bone Marrow Culture for the Diagnosis of Macrocytic Refractory Anaemias
Author(s) -
Garand R.,
Zittoun J.,
Marquet J.,
Mouraud E.,
Grolleau J. Y.,
Mevel A.,
Zittoun R.
Publication year - 1981
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1981.tb01394.x
Subject(s) - vitamin b12 , bone marrow , deoxyuridine , macrocytic anemia , medicine , in vitro , pathology , immunology , biology , gastroenterology , biochemistry , dna
Deoxyuridine (dU) suppression test and granulocyte macrophage colony forming unit (CFU‐GM) assay were performed, along with morphological analysis, vitamin B 12 and folate assays, in 22 cases of macrocytic anaemia corresponding to various types of idiopathic dysmyelopoietic syndromes. The dU suppressive effect was found to be normal in 19 cases, and slightly subnormal in 3 cases. The parallel study of the in vitro bone marrow growth showed, except in cases of primary acquired sideroblastic anaemia, a reduced number of colonies associated with a relative increase of the cluster formation. Consequently, these two parallel functional studies can support a formal diagnosis of potentially preleukaemic myelodysplasia, whatever may be the morphological pattern and even in cases of marked macrocytic anaemias and secondary epiphenomenal folate or vitamin B 12 deficiency.