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Monocyte Function in Normal Adults
Author(s) -
Lawton J. W. M.,
Gardner I. D.
Publication year - 1981
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1981.tb00456.x
Subject(s) - monocyte , function (biology) , chemistry , biology , medicine , immunology , microbiology and biotechnology
Human blood monocytes were separated by centrifugation over modified Ficoll‐Hypaque and by this one‐step procedure relatively high proportions (mean 54%) and yields of monocytes were obtained. Yields ranged from 0.22 times 10 9 to 0.98 times 10 9 /1 of blood and averaged 2 1/2 times the theoretical maximum yield calculated from the blood leucocyte and differential counts. A range of tests of monocyte function are described, namely, adhesion to glass, spreading on glass, chemotaxis under agarose and phagocytosis and killing of Candida albicans. These tests are generally simpler to perform than similar tests previously described and give very reproducible results which compare well with those reported by others. Normal values from 35 healthy Chinese adults are recorded.