Premium
Measurement of Antihaemophilic Factor A Antigen (VIII:CAg) with a Solid Phase Immunoradiometric Method Based on Homologous Non‐Haemophilic Antibodies
Author(s) -
Holmberg Lars,
Borge Lise,
Ljung Rolf,
Nilsson Inga Marie
Publication year - 1979
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1979.tb02847.x
Subject(s) - immunoradiometric assay , haemophilia , haemophilia a , antibody , chemistry , antigen , von willebrand disease , immunology , medicine , endocrinology , von willebrand factor , radioimmunoassay , platelet , surgery , biochemistry
Antihaemophilic‐factor‐A‐antibodies, which had spontaneously arisen in 2 patients, were used to develop an immunoradiometric method for measurement of antihaemophilic factor A antigen (VIII:CAg). 13 patients with severe haemophilia A had VIII:CAg below the limit of detection (0.01 U/ml). Patients with moderate and mild haemophilia A either had VIII:CAg roughly equal to factor VIII clotting activity (VIII:C) or a not detectable VIII:CAg, suggesting 2 different molecular mechanisms in moderate and mild haemophilia A. VIII:CAg could be detected in serum but in lower amounts than in plasma. In 2 patients with von Willebrand's disease VIII:CAg equalled VIII:C. The post‐transfusional retarded increase of VIII:C in 1 patient with von Willebrand's disease was accompanied by a slight increase in VIII:CAg. Fetal plasma contained measurable amounts of VIII:CAg.