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Studies on the Regeneration of the CFU‐C Population in Blood and Bone Marrow of Lethally Irradiated Dogs after Autologous Transfusion of Cryopreserved Mononuclear Blood Cells
Author(s) -
Nothdurft Wllhelm,
Bruch Christian,
Fliedner Theodor M.,
Ruber Esther
Publication year - 1977
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1977.tb01503.x
Subject(s) - bone marrow , peripheral blood mononuclear cell , haematopoiesis , andrology , medicine , colony forming unit , population , cryopreservation , granulocyte , immunology , biology , stem cell , embryo , biochemistry , genetics , environmental health , bacteria , in vitro , microbiology and biotechnology
In a group of 8 lethally irradiated (1200 R) dogs, that were transfused autologously with cryopreserved mononuclear cells (MNC) derived from the peripheral blood by leucapheresis the concentration of colony‐forming units in agar (CFU‐C) in bone marrow and peripheral blood was estimated at regular intervals after irradiation and transfusion of MNC. The numbers of MNC transfused per kg body weight ranged from 0.32 times 10 9 to 1.63 times 10 9 with an incidence of CFU‐C between 0.02 times 10 5 and 1.38 times 10 5 . In 6 dogs the CFU‐C levels in the bone marrow reached the normal pre‐irradiation values between days 15 and 20. But in 2 dogs that had received the lowest CFU‐C numbers the regeneration of the bone marrow CFU‐C was markedly delayed. In general the time course of the bone marrow repopulation by CFU‐C for single dogs was reflected by a corresponding regeneration pattern of the blood CFU‐C. The time course of the curves for the blood CFU‐C levels on the other hand was of the same kind as for the granulocyte values in the peripheral blood, that reached the normal levels mainly around day 30 and thereafter. Considerable fluctuations were seen in the blood CFU‐C levels of single dogs before irradiation and after mononuclear leucocyte transfusion. Despite of such limitations the blood CFU‐C content appeared to be a useful indicator of haematopoietic regeneration of the bone marrow.