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A simple HPLC method for the isolation and quantification of the allergens tuliposide A and tulipalin A in Alstroemena
Author(s) -
Christensen Lars P.,
Kristiansen Kell
Publication year - 1995
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.1600-0536.1995.tb00669.x
Subject(s) - high performance liquid chromatography , chromatography , distilled water , chemistry
A practical, rapid, reliable and sensitive method for the isolation and determination of the allergens tuliposide A and α‐methylene‐γ‐butyrolactone (tulipalin A), by reversed‐phase high‐performance liquid chromatography (RP‐HPLC), has been developed in order to select Alstroemeria species for breeding purposes. From the aqueous extracts of flowers, stems and leaves, of several Alstroemeria species, the contents of 6‐tuliposide A and tulipalin A were determined by isocratic RP‐HPLC, using distilled water as mobile phase. The compounds were detected by an UV detector at 208 nm. Differences in 6–tuliposide A and tulipalin A content were found among the species investigated, with the highest concentrations in stems and flowers. The absence of other tuliposides (e.g., 1‐tuliposide A, 1‐ and 6‐tuliposide B) in extracts was proven by TLC, RP‐HPLC, 1 H‐ and 13 C‐NMR. 6‐Tuliposide A and tulipalin A were identified by 1 H‐ and 13 C‐NMR and comparison with authentic material, respectively. With this HPLC method, it is possible to investigate a large number of plants for their contents of tuliposide A and tulipalin A, within a minimum of time, and to isolate them directly from aqueous extracts.