Premium
Treatment of experimental periodontal disease with antimicrobial photodynamic therapy in nicotine‐modified rats
Author(s) -
Garcia Valdir Gouveia,
Fernandes Leandro Araújo,
Macarini Valmir Campos,
Almeida Juliano Milanezi,
Martins Thiago Marchi,
Bosco Alvaro Francisco,
Nagata Maria José Hitomi,
Cirelli Joni Augusto,
Theodoro Letícia Helena
Publication year - 2011
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.2011.01785.x
Subject(s) - scaling and root planing , photodynamic therapy , antimicrobial , medicine , saline , ligature , periodontitis , adjunctive treatment , dentistry , chronic periodontitis , periodontal disease , chemistry , organic chemistry
Background The aim of this study was to compare antimicrobial photodynamic therapy ( aPDT ) as an adjunctive treatment to scaling and root planing (SRP) for induced periodontitis in nicotine‐modified rats. Material & Methods A total of 240 rats were evenly divided into two groups: C – saline solution treatment; N – nicotine treatment. Periodontal disease was induced in both groups at the first mandibular molar. After 7 days, the ligature was removed. All animals were submitted to SRP and were divided according to the following treatments: SRP – irrigation with saline solution; Toluidine Blue‐O (TBO) – irrigation with phenothiazinium dye (100 μg/ml); LLLT – laser irradiation (660 nm; 0.03 W; 4 J); and aPDT – TBO and laser irradiation. Ten animals in each group/treatment were euthanized at 7, 15 and 30 days. The histometric and immunohistochemical values were statistically analysed. Results Intragroup analysis demonstrated that in both groups the aPDT treatment resulted in lower bone loss (BL) when compared to SRP in all experimental periods. Intergroup analysis demonstrated that aPDT treatment resulted in lower BL in Group N than in Group C treated with SRP in all experimental periods. Conclusion Antimicrobial photodynamic therapy was an effective adjunctive treatment to SRP for induced periodontitis in nicotine‐modified rats.