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Lactobacillus casei prevents impaired barrier function in intestinal epithelial cells
Author(s) -
EUN CHANG SOO,
KIM YONG SEOK,
HAN DONG SOO,
CHOI JOO HYUN,
LEE A REUM,
PARK YOON KYUNG
Publication year - 2011
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1600-0463.2010.02691.x
Subject(s) - lactobacillus casei , barrier function , cytokine , intestinal epithelium , tumor necrosis factor alpha , protein kinase b , caco 2 , tight junction , biology , paracellular transport , mapk/erk pathway , chemistry , epithelium , microbiology and biotechnology , immunology , signal transduction , permeability (electromagnetism) , cell , biochemistry , genetics , membrane , fermentation
Eun CS, Kim YS, Han DS, Choi, JH, Lee AR, Park YK. Lactobacillus casei prevents impaired barrier function in intestinal epithelial cells. APMIS 2010 The exact effect of probiotics on epithelial barrier function is not well understood. The aims of this study were to evaluate cytokine‐induced epithelial barrier dysfunction in intestinal epithelial cells (IECs) and to study the role of probiotics in the prevention of epithelial barrier dysfunction. Caco‐2 cells grown on transwell chambers were stimulated with tumor necrosis factor (TNF)‐α or interferon (IFN)‐γ basolaterally. Probiotic, Lactobacillus casei , was added 1 h before cytokine stimulation. MAPK inhibitors were added 15 min before L. casei stimulation. The electrical resistance and paracellular permeability of Caco‐2 monolayers were measured. Distribution of zonula occludens (ZO)‐1 protein was assessed by immunofluorescence, and Western blot analyses for ZO‐1, p‐Akt, and toll‐like receptor (TLR) 2 were performed. Both TNF‐α and IFN‐γ stimulation on Caco‐2 cells decreased transepithelial resistance (TER), increased epithelial permeability, and decreased ZO‐1 expression of Caco‐2 cells. In contrast, pretreatment of L. casei reversed the cytokine‐induced dysfunction of TER, epithelial permeability, and ZO‐1 expression. Reversal of cytokine‐induced dysfunction of TER and intestinal permeability by L. casei was abrogated with MAPK inhibitor treatment. Lactobacillus casei stimulation on Caco‐2 cells increased TLR2 and p‐Akt expression. Probiotic, L. casei , prevents cytokine‐induced epithelial barrier dysfunctions in IECs.