Open AccessPeroxidatic activity in heart effluent: a biochemical parameter for the assessment of experimental ischemia‐reperfusion injury
Author(s) -
Leabu M.V.,
Bucur Elisa A.
Publication year - 2000
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2000.tb00115.x
Subject(s) - ischemia , reperfusion injury , myocardial reperfusion injury , medicine , cardiology , pharmacology
Peroxidatic activity in heart effluent was defined as a new biochemical parameter for the experimental study of myocardial ischemia. The peroxidatic reaction was determined by dot blot analysis with 3,3′‐diaminobenzidine as hydrogen donor. After ischemia, the level of peroxidatic activity in heart effluent was 2‐3 times higher than before. The effects in experimental modulation of ischemia, such as nicorandil or aprikalim protection, and the reversibility of protection by glibenclamide, could accurately be noted using the level of peroxidatic activity in heart effluent as a biochemical parameter. The results were in good agreement with those obtained for other enzymes used as biochemical parameters in experimental heart ischemia‐reperfusion studies.